Dissecting the functional role of APOBEC3B in multiple myeloma
Funding Cycle:
2022-2023Name:
Florian KuchenbauerType of Award:
Translational Research AwardHome Institution:
Terry Fox Laboratory, BC Cancer Research Institute, CanadaDescription
AIM 1 aims to identify APOBEC3B (A3B) targets and pathways affected in multiple myeloma (MM) cells. Using knockdown and overexpression techniques, researchers will assess the functional impact of A3B on MM cell viability, proliferation, apoptosis, and DNA damage. RNA-Seq and proteomics will provide mechanistic insights into A3B’s role in MM, potentially identifying affected pathways. AIM 2 involves studying A3B’s impact on MM progression in vivo using mouse models, complementing findings from human cell lines. AIM 3 focuses on targeting A3B activity with small molecule inhibitors, aiming to prevent drug resistance and tumor recurrence. Treatment with inhibitors will be evaluated in MM cell lines and mouse models, assessing cell behavior and disease progression. This comprehensive approach seeks to elucidate A3B’s role in MM and explore its therapeutic potential.
