Role of flow cytometry and especially its use in the definition of the measurable residual disease (MRD) in multiple myeloma
Basic Information
Funding Cycle :
2021-2022
Name :
Laura Notarfranchi
Type of Award :
Career Development Award
Home Institution :
University of Parma, Italy
Host Institution :
University of Navarra, Spain
Description
This project aims to develop a clinically useful method for monitoring treatment response in multiple myeloma (MM) by detecting circulating tumor cells in peripheral blood (PB) using next-generation flow cytometry. The proposed approach, called BLOOD-FLOW, involves optimizing immunomagnetic enrichment of circulating plasma cells from large PB volumes, achieving a limit of detection two logs more sensitive than current methods. Specific objectives include developing an efficient enrichment protocol and validating its sensitivity and specificity in PB samples from 50 MM patients post-treatment, comparing its performance to bone marrow minimal residual disease assessment.
